標(biāo)題: 現(xiàn)在的蛋雞、蛋種雞腫瘤病多么？ [打印本頁] 作者: 吠聲天外 時間: 2008-1-24 17:44 標(biāo)題: 現(xiàn)在的蛋雞、蛋種雞腫瘤病多么？ 查閱資料，驚見以下文字：
Avian leukosis virus (ALV) subgroup J (ALV-J) is an exogenous ALV and causes myeloid leukosis in meat-type chickens. We have previously reported the isolation and identification of ALV-J in commercial layer flocks from 12 farms in northern China. In this report, we further characterized this virus by in situ polymerase chain reaction (PCR) hybridization in various affected organs of chickens from six of the 12 farms. A routine method for hybridization of nucleic acid uses radioactive probe, such as a P(32)-labelled probe. We found that the non-radioactive digoxigenin (DIG) probe is sensitive enough to detect the nucleic acid of virus in chicken tissues. We used a pair of published primers (H5/H7) specific to the gp85 envelope gene and 3' region of pol gene of prototype ALV-J strain HPRS-103. The total RNA extracted from tumour, bone marrow, oviduct, liver and spleen of the diseased chickens from six commercial flocks, and cDNA was successfully amplified. Using the primers and cDNA, we obtained an ALV-J-specific cDNA probe of 545 bp in length by PCR. In situ PCR with H5/H7 primers was carried out in the paraffin sections from tissues of the diseased chickens, followed by in situ hybridization using the DIG-labelled cDNA probe. Positive hybridization signals were detected in the cytoplasm of paraffin sections of tumours and other organ tissues. The intensity of the signals was documented using an image analysis system measuring integral optical density (IOD). The IOD values for tissue sections treated by in situ PCR hybridization are significantly higher than that by in situ hybridization alone (P < 0.01). These data taken together suggest that in situ PCR hybridization is a more sensitive technique for detection of ALV-J in tissue sections.
目前飼養(yǎng)在我國北方的蛋雞、蛋種雞是否腫瘤多見？如果有腫瘤，是否腎臟腫瘤多見？另外，法氏囊、骨骼上是否有油脂樣增生物？
回帖者請注明品系和引種場家作者: zgsj016 時間: 2008-8-15 21:24
現(xiàn)比較多見。作者: tyslc 時間: 2008-8-16 06:44
現(xiàn)在有治療的方法嗎作者: 水無常色 時間: 2008-8-22 16:47
腫瘤多為病毒病，很難治療的。作者: bikaqiu 時間: 2008-8-24 14:41
腫瘤病對于雞來說就是癌癥，是不能治療的，只有通過源頭的凈化，應(yīng)用陰性雞去傳代生產(chǎn)。
不過有時候采用一些大蒜之類的藥物能否起到一點作用。作者: 孫蘇軍 時間: 2008-9-3 01:18
一直很多的，記得2000年解剖課共解剖了17只淘汰蛋雞，基本都有不止一個腫瘤，平均3個！搞得好幾天不吃雞肉